Purification and properties of acetyl coenzyme A synthetase from bakers' yeast.
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چکیده
منابع مشابه
Purification and properties of acetyl coenzyme A synthetase from bakers' yeast.
Acetyl-CoA synthetase, utilized in a coupled reaction system, has been shown to be applicable to the spectrophotometric determination of propionic and methylmalonic acids in biological fluids. The isolation of acetyl-CoA synthetase from yeast is simpler than the purification from mammalian sources. This study also presents some properties of the yeast enzyme and compares it to the more extensiv...
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Acetyl coenzyme A synthetase has been partially purified from many sources including yeast, bacteria, molds, plants, mammalian organs, and pigeon liver (5-11). Substrate amounts of this enzyme, partially purified by the method of Hele from beef heart mitochondria (lo), have been used for the isolation of acetyl adenylate from a reaction mixture containing all reactants except coenzyme A (4). Al...
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Since recent surveys of insect hemolymph (1, 2) have shown that trehalose is the major blood sugar, the hydrolytic enzyme trehalase has also awakened the interest of investigators. A purified preparation of the enzyme from Galleria mellonella (3) and from Phormia regina (4) was recently obtained and some of its properties described. The biosynthesis of trehalose with an enzyme preparation from ...
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Lysyl transfer ribonucleic acid synthetase (L-lysine: tRNA ligase (AMP), EC 6.1.1.6) was pursed to a state of apparent homogeneity from bakers’ yeast. For each of two different preparation procedures, a 250to 500-fold purikation of the enzyme was obtained, and the final purification step yielded two distinct protein components with specific activity values of approximately 0.5 and 1.0 pmole of ...
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Malonyl coenzyme A synthetase (EC 6.2.1.14) was induced in Pseudomonas fluorescens grown on malonate as a sole carbon source. This enzyme was purified, for the first time, over 30-fold by the combination of ammonium sulfate precipitation, Sephadex G-150 gel filtration, DEAE-Sephacel ion exchange chromatography, and hydroxylapatite chromatography. The purified enzyme, which had a specific activi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)32745-x